Journal: Japanese Journal of Clinical Oncology
Article Title: High-efficiency EGFR genotyping using cell-free DNA in bronchial washing fluid
doi: 10.1093/jjco/hyae021
Figure Lengend Snippet: (a). Specimen collection. Among lung carcinoma patients treated at the University of Tsukuba Hospital (Ibaraki, Japan) between 2015 and 2019, sBWF samples were obtained from 1159, and EGFR mutation testing (PNA-LNA PCR Clamp Method or Cobas EGFR Mutation Test v2) was undertaken for 310 using tissue samples (biopsy or surgical specimens). From among these cases, 80 were selected. Among them, the ex21 L858R EGFR point mutation was detected in 26 cases and the ex19 E746-A750 deletion mutation in 24; EGFR mutations were undetectable in 30 cases. Blood serum samples were included in three, three, and two cases, respectively. (b) Comparison of EGFR genotyping using cfDNA from supernatant of bronchial washing fluid (sBWF) with that using tissue samples (biopsy or surgical specimens). The positivity rate for EGFR mutation using cfDNA extracted from sBWF was 80% (40/50, ex21 L858R in 20/26 cases and ex19 E746-A750 del in 20/24 cases). Thirty cases were negative for EGFR mutation, but one case showed >2 copies of EGFR mutation/μL (ex21 L858R).
Article Snippet: Tissue samples were fixed with 10% neutral buffered formalin and used for histological diagnosis and EGFR mutation testing (PNA-LNA PCR Clamp Method or Cobas EGFR Mutation Test v2) (SRL Inc, Tokyo, Japan).
Techniques: Mutagenesis, Comparison